ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (8): 1246-1252.doi: 10.11843/j.issn.0366-6964.2014.08.006

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Sequencing Mitochondrial Cytochrome Oxidase Subunit of Tibetan Mastiff and Phylogenetic Analysis of Canidae

LI Yin-xia,QIAN Yong,CAO Shao-xian*   

  1. (Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
  • Received:2014-02-18 Online:2014-08-23 Published:2014-08-23

Abstract:

Based on the sequence of mitochondrial DNA Cytochrome oxidase subunit,the origin,taxon of Tibetan Mastiff and the phylogenetic relationship between Tibetan Mastiff and other large breed dogs were studied.Primers were designed according to the mitochondrial sequence of domestic dog,to amplify COI (Cytochrome oxidase subunit 1),COII (Cytochrome oxidase subunit 2) and COIII (Cytochrome oxidase subunit 3) of the Tibetan Mastiff.Phylogenetic trees among Tibetan Mastiff and other 19 breeds of Canidae were constructed using giant panda (Ailuropoda melanoleuca) as the outgroup.The results shown that the length of COI,COII and COIII were 1 545,684 and 784 bp,respectively,three genes coded 514,227 and 261 AA,respectively.Phylogenetic analysis displayed that the Tibetan Mastiff,12 domestic dog breeds and grey wolf clustered together,which indicated Tibetan Mastiff and other domestic dog breeds originated from grey wolf.In Canis,Tibetan Mastiff clustered together with Saint Bernard,Old English Sheepdog and Leonberger,but German Sheepdog,Swedish Elkhound and Black Russian Terrier clustered one group,middle and little breed dogs clustered together,which suggested that there were closer relationship between Tibetan Mastiff and Saint Bernard,Old English Sheepdog and Leonberger,it was confirmed that many large breed dogs in the world such as Saint Bernard were possible of having blood lineage of Tibetan Mastiff based on molecular data.The result indicate that Tibetan Mastiff originates from grey wolf,about the zoological taxon,Tibetan Mastiff belongs to Canis lupus familiarisCanis lupusCanisCanidaeCarnivora,the large breed dogs such as Saint Bernard probably have the blood lineage of Tibetan Mastiff in the breed forming.

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